A Novel Epitope from CD22 Regulates Th1 and Th17 Cell Function in Systemic Lupus Erythematosus
Figure 3
Identification for the specific anti-B2285 Abs.
(A) The flow cytometry analysis for the combination of anti-B2285 Abs to mouse B cells from MRL/lpr mice in PBS, anti-KLH Abs, anti-B2285 Abs and NB2285 (B2295 peptide neutralization Abs). (B) The analysis for the combination of anti-B2285 Abs to mouse CD4+ T cells from MRL/lpr mice by flow cytometry. (C) The detection of CD22 internalization mediated by preincubated with different Abs on viable B cells by flow cytometry. (D) The assay for the competitive binding to fixed B cells between anti-B2285 Abs and commercial anti-mouse CD22 Abs. The percentage of CD19+ CD22+ B cells was decreased obviously in B2285 group compared with those in PBS and KLH groups.