Ptc6 Is Required for Proper Rapamycin-Induced Down-Regulation of the Genes Coding for Ribosomal and rRNA Processing Proteins in S. cerevisiae
Figure 2
Ptc1 and Ptc6 differentially regulate the TOR signaling pathway.
A) Wild-type strain BY4741 and the indicated mutants were spotted onto YPD plates containing the specified concentrations of rapamycin. Growth was monitored after 3 days of incubation at 28°C. B) Exponentially growing BY4741 cells and the indicated mutants at a OD600 of 0.8–1.0 growing in YPD medium were incubated either with 200 ng/ml rapamycin (+) or the vehicle (−), for 6 h. Cells were then washed twice, resuspended in YPD, spotted onto YPD plates and the growth monitored after 3 days. C) The BY4741 strain and its ptc1 and ptc6 derivatives were transformed with an empty high-copy-number YEplac195 vector (YEp-Ø) or with the same vector bearing either the wild type or a catalytically inactive form of Ptc1 (PTC1[D58N]). Cells were spotted as described above and incubated for 3 days.