Selective Roles of Normal and Mutant Huntingtin in Neural Induction and Early Neurogenesis
Figure 2
Htt is required for the elaboration of FGF2-responsive dNSs, whereas mHtt differentially deregulates this process.
(A, B) Quantification of the size and number of KO and Q111 pNSs as compared to CTL and Q18 FGF2-responsive dNSs, respectively. Error bars represent ± SEM; unless otherwise stated, *p-value<0.05. (C, D) Quantification of the percentage of positive cells for proliferation markers, KI67 and pHisH3, and for the cell death marker, TUNEL, in KO FGF2-responsive dNSs as compared to CTL FGF2-responsive dNSs, and in Q111 FGF2-responsive dNSs as compared to Q18 FGF2-responsive dNSs, respectively. (E) Immunofluorescence micrographs of KI67 and pHisH3 immunoreactive cells in CTL, KO, Q18 and Q111 FGF2-responsive dNSs. (F, G) Quantification of the percentage of positive cells for the ESC marker, SSEA1, and the NSC marker, Nestin, in KO FGF2-responsive dNSs as compared to CTL FGF2-responsive dNSs, and in Q111 FGF2-responsive dNSs as compared to Q18 FGF2-responsive dNSs, respectively. (H) Immunofluorescence micrographs of SSEA1- and Nestin-immunoreactive cells in CTL, KO, Q18 and Q111 FGF2-responsive dNSs. (I) Quantification of the percentage of total positive cells for the early neuronal marker, β-TubIII, in CTL, KO, Q18 and Q111 FGF2-responsive dNSs. (J) Immunofluorescence micrographs of β-TubIII-immunoreactive cells in CTL, KO, Q18 and Q111 FGF2-responsive dNSs. Error bars represent ±95% CI; unless otherwise stated, *p-value<0.05. All scale bars = 25 μm.