Generation and Characterization of an Nxf7 Knockout Mouse to Study NXF5 Deficiency in a Patient with Intellectual Disability
Figure 4
Generation and genotyping of Nxf7 knockout mice.
(A) Schematic representation of the targeted Nxf7 allele (upper part) and its Cre-mediated KO allele (middle part). (B) Schematic representation of the Nxf7 transcript with the PCR primers. Position of exon 3 is indicated in dark gray. Amplicon sizes are indicated in base pair (bp). The agarose gel shows the PCR data of the expression analyses for the indicated primer pairs performed on brain cDNA of KO and WT male mice. (C) Western blot on yolk sac from WT and Nxf7 KO male embryos with the polyclonal Nxf7-Nt Ab. (D) Western blot on protein lysates obtained from transfected HEK-293T cells with the wild type Nxf7-flag cDNA (lane 1) and the exon3-lacking Nxf7-flag cDNA (lane 2). Detection was performed with anti-flag Ab. The anti-Actb Ab was used as the loading control. Western blot for the HA-tag gave the same result (data not shown).