Generation and Characterization of an Nxf7 Knockout Mouse to Study NXF5 Deficiency in a Patient with Intellectual Disability
Figure 1
Relative expression analysis of NXF/Nxf in tissues.
(A) RT-PCR for mRNA level quantification of Nxf1, Nxf2, Nxf3 and Nxf7 in mouse tissues. ActB was used as the housekeeping gene. (B) RT-qPCR for relative expression analysis of human NXF5 and NXF2 normalized to GUSB and HPRT1. (C) RT-qPCR for relative expression of mouse Nxf2 and Nxf7, normalized to Gusb and Hprt1. Cx, cortex; Hc, hippocampus; Cb, cerebellum; Te, testis; Br, Brain; Mu, muscle. N = 3. Means ± standard deviations. *p<0.05.