Regulation of Immune Responsiveness In Vivo by Disrupting an Early T-Cell Signaling Event Using a Cell-Permeable Peptide
Figure 3
R9-QQP specifically inhibits mucus production by goblet cells surrounding bronchioles.
(A) Representative micrographs (200×magnification) of bronchioles prepared by staining (PAS) cross-sections of lung tissue of mice that had been treated as indicated in Figure 2A and detailed in Materials and Methods. Inset boxes display additional digital magnification of corresponding region outlined in original image and red arrowheads indicate the presence of mucus producing goblet cells as defined by cytoplasm staining fuchsia. (B) Normalized averages (± SEM) of mucus severity scores for the indicated number (n) of mice calculated as described in Materials and Methods; for each mouse, approximately 30 bronchioles were stained and analyzed as in panel A. 100% mucus severity score correlated with a raw score of 3.3, assessed as described in Materials and Methods. *, p < 0.05; #, p>0.05.