Lactobacillus Decelerates Cervical Epithelial Cell Cycle Progression
Figure 6
The secretion of lactic acid reduces cell cycle progression.
The relative BrdU incorporation was measured in ME-180 cells in DMEM that has been adjusted with lactic acid to pH 7 or pH 6. The BrdU incorporation in DMEM (pH 7.4) was set to 100%. The means and standard deviations from five experiments are shown. (*indicates that p<0.05) (A). The graph indicates the pH levels that were measured in cell culture supernatants after ME-180 cells underwent 24 hours of incubation either alone or colonized with lactobacilli. The average pH values and standard deviations from 6 independent experiments are shown (and *indicates that p<0.05) (B). The relative BrdU incorporation of ME-180 cells that have been exposed for 24 hours to sterile-filtered cell culture supernatants originating from 24 hours of incubation of ME-180 cells either alone or colonized with lactobacilli. The BrdU incorporation level of the cells incubated with the supernatant from ME-180 cells alone was set to 100%. The averages and standard deviations from 3 independent experiments are shown (*indicates that p<0.05) (C).