Angiotensin II Increased Neuronal Stem Cell Proliferation: Role of AT2R
Figure 3
Ang II -induced ERK and Akt activation.
A) Representative blots (upper panel) and mean data of relative blot density (lower panel) showing Ang II induced a rapid phosphorylation of ERK and Akt in NSCs. Cells were treated with Ang II for different time followed by western- blot analysis. *P<0.05 vs untreated group; n = 3 in each group. B) Representative blots (upper panel) and mean data of blot density (lower panel) showing blockade of AT1R and AT2R on phosphorylation of ERK and Akt. NSCs were pre-treated with specific antagonist to AT1R or AT2R for 1 hour followed by incubation with Ang II for 30 min. *P<0.05 vs untreated group, #P<0.05 vs Ang II-treated group; n = 3 in each group. C) Representative blots (upper panel) and mean data of relative blot density (lower panel) showing CGP42112A induced a rapid phosphorylation of ERK and Akt in NSCs. Cells were treated with CGP42112A for different time followed by western-blot analysis. *P<0.05 vs untreated group; n = 3 in each group. D) Effect of blockade of ERK and Akt on Ang II-induced NSC proliferation. Cells were pretreated with specific ERK or Akt pathway inhibitor-U0126 or LY2940002 for 1 hour followed by treatment with Ang II for another 48 hours. Cell proliferation was assessed by CyQUANT Assay. *P<0.05 vs untreated group, #P<0.05 vs Ang II- treated group; n = 5 in each group.