The Role of Interleukin-1 and Interleukin-18 in Pro-Inflammatory and Anti-Viral Responses to Rhinovirus in Primary Bronchial Epithelial Cells
Figure 6
IL-1 is required for the HRV-dependent release of pro-inflammatory cytokines and neutrophil chemoattractants but not T-cell chemoattractants or members of the IFN family.
NHBE cells were uninfected, infected with HRV14 or infected with HRV14 in the presence of anakinra (10 nM). After the indicated time RNA was extracted and changes in mRNA was determined by a microfluidic genecard. The data from CSF2 (A), CXCL2 (B), CXCL11 (C) or IFNB1 (D) are presented and is expressed as Mean (± SEM) fold expression (2−ΔΔCt) in relation to the untreated controls (A–C) or the HRV14 treated samples (D) (N = 3). An * indicates a significant increase in mRNA expression on treatment with HRV14 compared to untreated NHBE cells, a # indicates a significant decrease in mRNA expression when the infected NHBE cells were treated with anakinra. An ns indicates not significant, (***/# # # p<0.001, **/# # p = 0.001–0.01, */# p = <0.05).