Methylated BSA Mimics Amyloid-Related Proteins and Triggers Inflammation
Figure 1
mBSA displays features of amyloid.
(A) Fluorescence intensity of Thioflavin T at 480 nm in buffer, with BSA, mBSA, Aβ or with the reverse control peptide. Error bars are means ± SD of 3 independent experiments. **p<0.005. (B) Gel shift analysis of salmon sperm ssDNA pre-mixed with different amounts of BSA or mBSA. (C) Thioflavin S fluorescence in the presence of different BSA proteins or aggregates observed under fluorescence microscopy. Original magnification 100X. scale bar: 20 µm. (D) Fluorescence emission profiles of Congo Red in the presence of buffer, different proteins or aggregates. (E) Birefringence of Congo Red on mBSA or mBSA plus ssDNA aggregate observed by polarized light microscopy. Original magnification 40X. bar: 50 µm. (F) Transmission electron microscopy analysis of mBSA or mBSA plus ssDNA. Bar: 100 nm.