The Drosophila Cadherin Fat Regulates Tissue Size and Planar Cell Polarity through Different Domains
Figure 5
The H2 region is necessary but not sufficient for regulating the Hpo/Wts pathway.
ftG−rv mutant clones, marked by the absence of GFP expression (green channel), were generated in third-instar eye imaginal discs in which Ft mutant transgenes were expressed by TubP-Gal4. DIAP1 staining was shown in red. White arrows point to ft mutant clones. (A) ft mutant clones show autonomous increases in DIAP1. Ubiquitous expression of Ft-FL (B), Ft-intra (C), MH2345 (D), and FL-ΔH345 (F) rescues the elevated DIAP1 levels in ft clones, whereas MH2 (E) does not rescue (see result section). (G) Quantification of the rescue of DIAP1 expression in ft clones. The plot shows the percentage of rescued clones observed with the indicated different Ft mutant transgenes. (H) Quantification of the rescue of clonal overgrowth in ft clones. The plot shows the percentage of rescued clones observed with the indicated different Ft mutant transgenes.