The Drosophila Cadherin Fat Regulates Tissue Size and Planar Cell Polarity through Different Domains
Figure 1
Summary of Ft constructs and their ability to rescue ft mutant phenotype.
Schematic structure of Ft mutant constructs is on the left with their short names. (See Materials and Methods for details.) Summary of rescue results is on the right. Measurement and quantification of each rescue assay can be found in indicated figures. In the lethality rescue assay, effective rescue (+) was determined by at least 25% recovery of the expected number of ftG−rv/ftl(2)fd; TubP-Gal4/UAS-transgene adults, whereas no rescue (−) was determined for those transgenes which did not produce any adult animal (eclosion). In the eye disc size rescue assay, effective rescue (+) was determined by <20% increase of size compared to wt disc. In the PCP rescue assay, effective rescue (+) was determined by >85% correct orientation of ommatidia; (−) indicates disorganized polarity that the equator was unable to be recognized, and <75% correct orientation can be determined. In the clonal DIAP1 and clone size assays, effective rescue (+) was determined by >60% of clones with successful rescue of clonal phenotype and DIAP1 expression level. ‘DN’ indicates dominant negative effect of FL-ΔH2. N.D.: not determined.