Development of Autoimmune Hair Loss Disease Alopecia Areata Is Associated with Cardiac Dysfunction in C3H/HeJ Mice
Figure 7
qPCR analysis of atria treated with ACTH for 72 hours.
For atria derived from AA mice, Il18 expression was highest at 1 µM of ACTH (A); the expression of Casp1 showed similar trend (D). Il18r1 expression was dependent on ACTH concentration (B) while Il18bp was down-regulated (C). For atria derived from sham-grafted mice, Il18 expression was lowest at 1 µM unlike AA (E). However, Il18bp was significantly increased at 1 µM (G). ACTH had a significant effect on the control atria with concentration dependent increase of Il18r1 (F) and Casp1 (H). Gene expression levels were calculated as fold change compared to no-treatment control (0 µM ACTH). Error bars represent the range factor difference (2−ΔΔCt±ΔCtSD). Statistical significance determined with Student’s t-test; *denotes p<0.05.