Cloning, Expression and Characterization of 3-Hydroxyisobutyrate Dehydrogenase from Pseudomonas denitrificans ATCC 13867
Figure 1
Analysis of relative mRNA levels for 3hibdhI, 3hibdhII, 3hibdhIII and 3hibdhIV in wild type P. denitrificans ATCC13867.
P. denitrificans ATCC13867 was cultured in M9 medium without (black bar) or with 3-HP supplementation (grey bar) at 30 mM and harvested in exponential growth period. The mRNA levels were estimated with rpoD, encoding sigma factor 70, as reference gene. The values are average of four experiments.