Fine Mapping and Functional Analysis of the Multiple Sclerosis Risk Gene CD6
Figure 4
CD6 expression on different cell types segregated by non-synonymous CD6 haplotypes, as analyzed by FACS.
A total of 27 PBMC samples representing each of the three haplotypes from the two non-synonymous SNPs rs11230563 and rs2074225 were analysed for CD6 expression on the different cell subsets. The y-axis represents the median fluorescence intensity (MFI) while the x-axis represents the three different haplotypes; i.e. the CC haplotype that confers protection, CT associated with risk and TT conferring mild risk. The distinct subpopulations were identified using different surface markers as listed in Table S4. The markers CD3 and CD56 were used to identify T cells (CD4+/CD8+ CD3+ CD56−), NK (CD3− CD56+), and NKT (CD3+ CD56+) cell subsets; CD45RA, CD27 and CD28 were used to distinguish naïve (CD45RA+ CD27+ CD28+), central memory cells (CD45RA− CD27+) and effector memory cells (CD45RA− CD27− CD28+), as well as the terminally differentiated effector memory cells (TEMRA, CD45RA+ CD27− CD28−); while CD56 and CD16 were used to identify the NK CD56dim (CD56int CD16+) and the CD56bright (CD56hi CD16) cells.