General Cell-Binding Activity of Intramolecular G-Quadruplexes with Parallel Structure
Figure 2
Cellular binding (A) and CD spectra (B) of F-PQSs treated in different buffers.
F-PQSs were heat-denatured and annealed in buffers with or without 150 mM Na+ and 10 mM K+ respectively before dilution for measurement. For flow cytometric assay, final concentration of F-PQSs was 0.4 µM; cell line, K562. The inset histograms represent the geometric mean fluorescence of cells after deducted the auto-fluorescence. For CD spectra measurement, the final concentration of F-RET, F-c-Myc and F-c-Kit2 was 4 µM; F-VEGF was 2.6 µM.