Measuring EGFR Separations on Cells with ∼10 nm Resolution via Fluorophore Localization Imaging with Photobleaching
Figure 6
Using confidence intervals as diagnostics.
(A) A sum of the individual bootstrap separation densities. The left-hand side shows a cartoon of the separation distribution of a single two-fluorophore spot. The right-hand side shows the distribution of the combined separation data from multiple two-fluorophore spots. (B) A 1sCI-Plot (right) is the scaled sum of top hat functions (left). Each top hat is created from the bootstrap separation distribution of a two-fluorophore spot. The top hat - or indicator - function has the value 1 if the separation lies within the 68% confidence interval, otherwise it has the value 0. The 1sCI-Plot plot (right) is an example sum of such functions for multiple two-fluorophore spots, where the scaling consists of dividing by the number of spots and then multiplying by 100 for a % scale. Hence, a value of 50% at a particular separation means that separation lies in the 68% confidence interval of the separation distribution of half of the two-fluorophore spots included in the analysis. The black line shows the ideal peak value (68%) expected for a repeated measurement of a single distance and the dashed lines show the uncertainty of this ideal due to the limited number of samples. (C) A 2sCI-Plot is the scaled sum of crosses, all with the same height and with equal arm widths given by the 68% confidence limits of each individual bootstrapped separation.