Alpha-Synuclein Induces Lysosomal Rupture and Cathepsin Dependent Reactive Oxygen Species Following Endocytosis
Figure 8
Large aggregates generated by the E46K mutant of α-synuclein do not induce vesicle rupture or ROS as efficiently as wt α-synuclein.
(A) SH-SY5Y or (B) N27 cells were loaded with H2DCFDA reagent and incubated with wt or E46K α-synuclein. Error bars represent the SEM of triplicate samples. Results are representative of at least three independent experiments. C. Purified recombinant E46K α-synuclein aggregates were added to N27 chGal3 cells for 24 hours, stained with an α-synuclein specific antibody (green) and imaged as in previous experiments. Results are representative of at least three independent experiments.