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The Response of Human Macrophages to β-Glucans Depends on the Inflammatory Milieu

Figure 3

Production of PGE2.

(A, C, E and F) Macrophages were primed with 10 ng/ml LPS or (B) 100 U/ml IFNα and IFNγ for 3 hours or left untreated, and then stimulated with different additions at a concentration of 1 mg/ml. 10 µM CA-074, 2 µM cytochalasin D, and 20 µg/ml PVNO were added before the stimuli. The supernatants were collected for the assay of PGE2. Results represent mean ± S.D. of 6 to 7 independent experiments. Veh, indicates vehicle, *indicates p<0.05.

Figure 3

doi: https://doi.org/10.1371/journal.pone.0062016.g003