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An Image Analysis Algorithm for Malaria Parasite Stage Classification and Viability Quantification

Figure 3

Parasite signal detection process diagram.

(A) DAPI or Mitotracker fluorescence channel image. (B) Detect local maxima points of intensity. (C) Compute and of local maximum point intensities. (D) Smooth signals by Gaussian filtering. (E) Separate signals by threshold value . (F) Compute number, location, area, signal strength information.

Figure 3

doi: https://doi.org/10.1371/journal.pone.0061812.g003