Non-Thermal Radio Frequency and Static Magnetic Fields Increase Rate of Hemoglobin Deoxygenation in a Cell-Free Preparation
Figure 1
EMF effect on hemoglobin (Hb) visible light spectra.
Spectra representative of the effects of pulsed radiofrequency (PRF) signal and 186 mT static magnetic field (SMF) on Hb visible light spectra during bHb)deoxygenation. Data shown are typical of samples drawn from a single tube for each EMF exposure condition, shown here at 150 min after a single 30 min EMF exposure. Deoxygenation of 100 µM Hb was carried out in 50 mM Hepes buffer (pH 7.2) using the reducing agent dithiothreitol (20 mM) at 22°C, and is characterized here by the passage of the spectrum from a two-peaked to one-peaked form. Deoxygenation occured at an earlier time for EMF exposed samples (traces with one peak), as compared to control samples exposed only to the ambient geomagnetic laboratory environment (trace with two peaks). The EMF effect was observable at the time of most rapid deoxygenation.