Identification of a Live Attenuated Vaccine Candidate for Tularemia Prophylaxis
Figure 8
Immunization with the FTL_0325 mutant results in a potent memory recall response.
Cell culture supernatants collected from BMDMs infected with either the F. tularensis LVS (A–D) or F. tularensis SchuS4 (E and F) and co-cultured with the splenocytes prepared from immunized mice at day 45 post-immunization (A and C) or at day 60 (B–F) were analyzed for IFN-γ (A, B and E) and IL-17a (C,D and F) cytokines by Cytometric Bead Flex sets. The data are representative of two independent experiments and were analyzed using ANOVA with Tukey-Kramer Multiple Comparison post-test and P values recorded. *P<0.05; **P<0.01; ***P<0.001.