Enhanced Immune Response and Protective Effects of Nano-chitosan-based DNA Vaccine Encoding T Cell Epitopes of Esat-6 and FL against Mycobacterium Tuberculosis Infection
Figure 4
The CTL activity of the mice elicited by nano-Esat-6/3e-FL or other plasmids.
Splenocytes from naive mice pulsed with (CFSEhigh) or without (CFSElow) peptides were transferred into the immunized mice. The representative histograms (A) and percentages (B) of specific lysis in the immunized mice were compared. Data are one representative results from three performed experiments, and presented as the mean ± SD (n = 6). NS: P>0.05; *P<0.05; ***P<0.001 versus the mice treated with nano-chitosan, nano-pIRES or nano-FL plasmids; the other differences among nano-Esat-6/3e-FL, nano-Esat-6/3e, Esat-6/3e-FL, nano-Esat-6, nano-Esat-6-FL and BCG treatments were showed on the figure directly.