SIMPL Enhancement of Tumor Necrosis Factor-α Dependent p65-MED1 Complex Formation Is Required for Mammalian Hematopoietic Stem and Progenitor Cell Function
Figure 7
SIMPL enhances the appearance of p65, MED1 and serine 5 phosphorylated RNA pol II on the Tnf gene promoter.
(A) Schematic of the mouse Tnf gene indicating location of PCR primers. (B and C) MEFs derived from littermate controls (WT) or SIMPL−/− mice were left untreated (-) or were treated with rhTNFα (10 ng/ml) for 45 minutes. Chromatin immunoprecipitation (ChIP) assays were performed as described under Materials and Methods with the indicated antibodies and primer sets specific for the mouse Tnf gene (see Panel A for location of primers; Materials and Methods for sequence of primers). ChIP assays were repeated at least three times.