Ammonium Inhibits Primary Root Growth by Reducing the Length of Meristem and Elongation Zone and Decreasing Elemental Expansion Rate in the Root Apex in Arabidopsis thaliana
Figure 4
Ammonium does not alter the root stem cell activity, but reduced columella cell number.
(A) Confocal fluorescence micrographs of PI-stained root tips taken at five days after treatment. WOX5::GFP marks the quiescent center specific marker; J2341 marks columella stem cell. Images are representative of 12–16 plants in four replicate experiments treated as in Figure 1A. Bar = 50 µm. (B) Bright-field micrographs taken at five days after treatment. DR5::GUS marks response to endogenous auxin; Lugol staining marks amyloplasts; and CS31333 marks mature columella cells. Images are representative of 12–16 plants in four replicate experiments treated as in Figure 1A. Bar = 50 µm.