Upregulation of miR-150* and miR-630 Induces Apoptosis in Pancreatic Cancer Cells by Targeting IGF-1R
Figure 3
Overexpression of pre-miR-150* reduced target proteins and induced apoptosis in pancreatic cancer cells.
(A) Increased expression of pre-miR-150* expression vector and pre-miR-150* reduced expression of c-Myb and IGF-1R mRNA and protein in pre-miR-150* stably transfected cells (B–D). Cells were treated with 1 µM 3-Cl-AHPC for 24 h. (E) pre-miR-150* induced apoptosis by itself and enhanced 3-Cl-AHPC mediated apoptosis in PANC-1 cells. Cells were transiently transfected with pre-miR-150* expression vector for 24 h and then exposed to 3-Cl-AHPC for 24 h. Induction of apoptosis was assessed using Annexin V-FITC labeling with propidium iodide (PI) staining in cells and the error bars represent the mean of three separate determinations ± the standard deviation (SD). All treated samples are significantly different from miR-vector. * (<0.05), ** (<0.01 ) and *** (<0.001 ) significantly different in comparison to miR-vector by t-Test. Over-expression of miR-150* induced activation of caspase 3 as indicated by cleaved caspase 3 fragments in pre-miR-150* transiently transfected cells (upper right panel). pre-miR-150* expressed PANC-1 cells induced apoptosis as indicated in acridine orange/ethidium bromide stained cells in lower right panel. Cells were transfected with pre- miR-150* for 48 h and then stained and photographed using Olympus fluorescence microscope digital camera software and DP2-BSW software.