Hydroxysteroid Sulfotransferase SULT2B1b Promotes Hepatocellular Carcinoma Cells Proliferation In Vitro and In Vivo
Figure 1
SULT2B1b promotes the growth of mouse hepatocarcinoma cells in vitro.
The mRNA (A) and protein (B) levels of SULT2B1 in transduced Hepa1-6 cells were detected by qPCR and western blot analysis respectively. SULT2B1 sulfotransferase activity in transduced Hepa1-6 cells was detected by sulfotransferase assay in vitro (C) or by measuring the conversion rate of [3H] cholesterol to [3H] methanol-water-soluble counts by adding [3H] cholesterol to live cells (D). SULT2B1b protein expression in Hepa1-6 cells transduced with Ad-EGFP or Ad-SULT2B1b (E). CCK-8 assay of the growth curves of Hepa1-6 cells transduced with SULT2B1-RNAi-LV, Ad-SULT2B1b, or vector controls (F,G). *represents P<0.05 vs. Ad-GFP group or NC-GFP-LV group.