Protection of Cells against Oxidative Stress by Nanomolar Levels of Hydroxyflavones Indicates a New Type of Intracellular Antioxidant Mechanism
Figure 6
Cytotoxicity of flavones in myoblasts.
Effects of the four hydroxyflavones on the survival of L-6 cells, as determined by the MTT assay. The concentration of cumene hydroperoxide was 27.5 µM, since preliminary experiments showed that higher concentrations were too toxic for these cells, and incubation with MTT was carried out for 24 h. Data are given as optical density (O.D.) rather than viability, because the values measured also include a component due to proliferation of the myoblasts. The bar graph shows data from a representative experiment with measurements carried out in triplicate. Data are mean ± SD of 3 different experiments. #not statistically different from Control; **p<0.01 with respect to cumene hydroperoxide alone.