Protection of Cells against Oxidative Stress by Nanomolar Levels of Hydroxyflavones Indicates a New Type of Intracellular Antioxidant Mechanism
Figure 3
Dose-dependence curves for elimination of ROS by 5,6-dihydroxyflavone.
Wide concentration range dose-responses of the antioxidant activity of 5,6-dihydroxyflavone in THP-1 monocytes (Upper panel) and in L-6 myoblasts (Lower panel). The oxidative stress was induced and measured as in Fig. 2. In addition to monocytes (▪) the upper panel also includes results obtained for differentiated macrophages after PMA treatment for 24 h (▴) or 72 h (▾). Data are reported as mean ± SD of 5–10 different experiments. p<0.05 at least, as from a Student's t test, starting from 10−10 M for both L-6 cells and THP-1 cells, at any stage of differentiation.