A Novel Alkaliphilic Bacillus Esterase Belongs to the 13th Bacterial Lipolytic Enzyme Family
Figure 5
Effect of pH on the activity of esterase EstOF4.
The reaction was taken at 50°C with pNC6 as substrate in different pH solution: sodium phosphate (pH 7.0 to 8.0), Tris-HCl (pH 8.0 to 9.0), glycine-NaOH (pH 9.0 to 10.5) and Na2HPO4/NaOH (pH 11.0 to 11.5). Each value of the assay was the arithmetic mean of triplicate measurements. Where pH overlapping, the averaged values were used.