Ionotropic Crustacean Olfactory Receptors
Figure 3
Spiny lobster IR transcripts can be detected in single ORN clusters.
RT-PCR detection of IRs in RNA prepared from (top panel) total olfactory tissue and (bottom panel) a single ORN cluster. While all of the IRs tested could be detected in the total RNA sample, only a limited number could be detected in the single cluster. No amplification was detected in RNA samples in the absence of reverse transcription or template (not shown). Numbers above panels indicate the IR amplified in the wells below. X indicates empty well.