Impact of E1 and Cre on Adenovirus Vector Amplification: Developing MDCK CAV-2-E1 and E1-Cre Transcomplementing Cell Lines
Figure 4
Levels of mRNA of E1A and E1B over increasing cell culture passages for MDCK-E1 clone #106 (a) and #139 (b).
The gene expression was determined by Real Time reverse transcriptase PCR normalized to the housekeeping gene (GAPDH). Error bars represent a 10% variability error associated with the method.