A Novel Peptide Delivers Plasmids across Blood-Brain Barrier into Neuronal Cells as a Single-Component Transfer Vector
Figure 2
RDP delivers plasmid pVAX-BDNF into the brain and gene expression.
(A), Gel retardation assay tests the migration pattern of plasmid DNA (2 µg) in the presence of RDP. The lanes 1 to 6 correspond to retardation assays of 4, 2, 1, 0.5, 0.25, 0.125 µg of RDP; lane 7, DNA markers. (B), Zeta potential of RDP/pVAX-EGFP complexes of different gram ratios (n = 3). (C), Serum stability of peptide and plasmid complexes. Time points were analyzed at 0, 2, 4, 8 and 12 h following incubation with mouse serum at 37°C. (D), Western blot analysis of the BDNF in brain, kidney and liver of mice. The mice were intravenously injected with 40 µg complexes of DNA and plasmid, and the BDNF levels were detected on day 0. 1, 3, 5, 7 after injection. (E), Transient decrease of BDNF level in the brain. Data were expressed as mean ± SEM (n = 3 in mice/time point). *p<0.05, **p<0.01 compared to the control (day 0),