Mechanisms of Adhesion and Subsequent Actions of a Haematopoietic Stem Cell Line, HPC-7, in the Injured Murine Intestinal Microcirculation In Vivo
Figure 1
HPC-7 adhesion in vitro and in vivo is increased on IR injured intestine.
(A) HPC-7 adhesion in vitro was raised on frozen ileal sections isolated from IR injured animals when compared to sham controls. Results represent mean adhesion per 1×104 µm2 tissue area±SEM (n>4/group); * p<0.05. (B) HPC-7 adhesion in ileal mucosal microcirculation in vivo was also raised in animals subjected to IR injury (solid line: sham; dashed line: IR injury). (C) Free-flowing HPC-7 cells were increased in IR mice (solid line: sham; dashed line: IR injury). (D) IR injury enhanced HPC-7 adhesion within jejunal villi when quantitated ex vivo. (E) IR injury did not enhance HPC-7 adhesion to duodenum when quantitated ex vivo. Representative images of the villous microcirculation of the ileum in (F) sham and (G) IR animals are shown. Results are presented as mean adhesion±SEM (n≥7/group); * p<0.05. For frozen section work, data is expressed as mean adhesion per 1×104 µm2 of tissue to control for area variation.