Transcriptome and Biochemical Analysis Reveals That Suppression of GPI-Anchor Synthesis Leads to Autophagy and Possible Necroptosis in Aspergillus fumigatus
Figure 7
Constructions of the QutG deletion mutant and PtdIns3K over- expression strain in A. fumigatus.
The null mutant ΔqutG (A) and PtdIns3K overexpression strain OEpi3k (C) were constructed and confirmed as described under Materials and Methods. After growing in liquid CM at 37°C for 12 h (D) or 24 h (B), the mycelia were stained with propidium iodide (PI) and examined under the fluorescene microscope (Zeiss).