A Novel Synthetic Microtubule Inhibitor, MPT0B214 Exhibits Antitumor Activity in Human Tumor Cells through Mitochondria-Dependent Intrinsic Pathway
Figure 3
MPT0B214 induces G2-M phase arrest and change in the expressed and phosphorylated status of G2-M regulators, followed by cell apoptosis in KB cells.
(A) The time effect of MPT0B214 on cell cycle distribution in KB cells (inserted figure: DNA histogram). Cells were treated with 10 nM MPT0B214 for different time points and analyzed for PI-stained DNA content by flow cytometry (B) Change in the expressed phosphorylated status of G2-M regulators under treatment. Cells are harvested after MPT0B214 treatment for 24 h, and the cell extract was prepared and loaded on SDS-PAGE. After electrophoresis, proteins were transferred to blots and probed with cyclin B1, Cdc25C, pCdc2, MPM2, and an internal control, α-tubulin antibodies.