Effect of 2-Hydroxyethyl Methacrylate on Antioxidant Responsive Element-Mediated Transcription: A Possible Indication of Its Cytotoxicity
Figure 7
Effect of MMA and HEMA on Gin-1 cells.
(A) Effect on intracellular GSH levels. Gin-1 cells were incubated for 6 h without or with indicated initial concentrations of MMA or HEMA, and subjected to the assay for intracellular levels of GSH. Data are presented as the mean ± SD (n = 3). (B) Effect on cell viability. Gin-1 cells were incubated for 24 h without or with indicated initial concentrations of MMA or HEMA, and subjected to the assay for cell viability using WST-8. Cell viability is expressed with the control value, obtained after incubation without exposure to MMA or HEMA, taken as 100%. Data are presented as the mean ± SD (n = 3). (C) Effect on promoter activity in Gin-1 cells transfected with the vector pGL4.24-2E. Gin-1 cells were co-transfected with pGL4.24-2E and phRL-CMV, cultured for 24 h, incubated for 6 h without or with MMA or HEMA (10 mM each), and subjected to the assay for the luciferase activities. Relative luciferase activities were expressed with the control value, obtained after incubation without exposure to MMA or HEMA, taken as 1.0. Data are presented as the mean ± SD (n = 3–8). *p < 0.05, **p < 0.01.