Osbpl8 Deficiency in Mouse Causes an Elevation of High-Density Lipoproteins and Gender-Specific Alterations of Lipid Metabolism
Figure 4
Western blot analysis of HDL apolipoproteins in WT and KO mouse plasma and high-density lipoprotein fractions.
A. Chow diet-fed animals; ApoA-II, ApoC-III, and ApoE (identified on the right) in the fasting plasma (Pla) of WT and KO mice of both genders (identified at the top); Bottom panel, ApoE in the HDL peak fractions of plasma fractioned on a Suprose HR 6 10/30 column. B. Western diet-fed animals; ApoE in the plasma (Pla) and HDL fractions (HDL) of WT and KO mice of both genders (identified at the top). The plasma loading was 3 µl/lane of 1/40 dilution, and that of Superose 6 HR 10/30 HDL fractions 150 µl/lane concentrated by acetone precipitation. The analysis was carried out for pooled plasma and HDL fractions isolated from the same pools; Chow diet, 3 animals/pool; Western diet, 6 animals/pool.