Cholesterol Biosynthesis and Homeostasis in Regulation of the Cell Cycle
Figure 6
Lovastatin or triparanol does not alter cell cycle distribution in the presence of additional serum cholesterol.
Both lovastatin (2.5 µM) and triparanol (7.5 µM) treatment of F111 cells resulted in the arrest of cells in G1 phase (blue bars) of cell cycle in presence of 10% serum (shown in panel (A)). Interestingly, presence of additional serum cholesterol (i.e., DMEM supplemented with 20% serum) abolished the G1 arrest of cells during cell cycle progression (see panel (B)). Cell numbers in S and G2 phases are represented by maroon and cyan bars, respectively. Values represent means ± SE of at least four independent experiments. See Materials and Methods for more details.