The Role of Siglec-1 and SR-BI Interaction in the Phagocytosis of Oxidized Low Density Lipoprotein by Macrophages
Figure 2
Siglec-1 knockdown can attenuate oxLDL uptake by macrophages.
BMMs were cultured for 6 days and transfected with mock siRNA (C) or siRNA targeting Siglec-1 (D, E, H), 24 h later, oxLDL 100 µg/ml (B∼E) or LDL 100 µg/ml (G∼H) was added and stimulated for 48 h. Then Oil red O staining was performed (A∼E, G∼H). Red particles in the cytoplasm indicated Oil red O positive lipid. A, control; B, oxLDL 100 µg/ml; C, mock transfection; D, m-si 2; E, m-si 3. Magnification ×400 (A–E). (F), Statistical analysis of A–E, randomly 20 highpower fields were selected in each group. #p>0.05, ***p<0.001. (G), native LDL 100 µg/ml was used as control. (H), LDL 100 µg/ml+m-si 3. Magnification ×1000 (G–H). (I), Statistical analysis of G–H. #p>0.05. (J), Quantitation of Oil red O staining in B–E with a spectrophotometric method. #p>0.05, *p<0.05. (K), Quantitation of Oil red O staining in G–H. #p>0.05.