Monocyte Chemoattractant Protein-1/CCL2 Produced by Stromal Cells Promotes Lung Metastasis of 4T1 Murine Breast Cancer Cells
Figure 1
The expression of MCP-1 and CCR2 by 4T1 cells.
A. The expression of MCP-1 mRNA by 4T1 cells was examined and compared to that of LLC by Northern blotting. B. The concentration of MCP-1 in the culture supernatants of 4T1 or LLC cells incubated for 24 h with 1 or 10 ng/ml of murine TNFα or 100 ng/ml of LPS or without any stimulus was measured by ELISA. C. The surface expression of CCR2 on 4T1 cells was examined by FACS. D. The expression of CCR2 mRNA in 4T1 cells was examined by RT-PCR. Thioglycollate-induced mouse peritoneal exudates cells were used as control.