Involvement of DNA-PKcs in the IL-6 and IL-12 Response to CpG-ODN Is Mediated by Its Interaction with TRAF6 in Dendritic Cells
Figure 2
Loss of DNA-PKcs impairs phosphorylation of IKKβ, IκBα, NF-κB and JNK in response to CpG-ODN.
(A) WT, DNA-PKcs−/− (PKcs−/−), TLR9−/− and PKcs−/−/TLR9−/− DCs were treated with CpG (5 µg/ml) for the indicated time durations or left untreated. Cell lysates were prepared and phospho-IKKα/β and beta-actin were detected by an immunoblotting (IB) analysis. (B) WT, DNA-PKcs−/− (PKcs−/−), TLR9−/− and PKcs−/−/TLR9−/− DCs were treated with CpG (5 µg/ml) for the indicated time durations or left untreated. The phospho-IκBα, phospho-NF-κB and phospho-JNK as well as JNK and beta-actin were detected. Similar results were obtained from at least three independent experiments.