Nicotinic Receptor Alpha7 Expression Identifies a Novel Hematopoietic Progenitor Lineage
Figure 7
Production of inflammatory and anti-inflammatory cytokines by spleen cells of recipient mice reconstituted with both α7lin+ and α7lin– cells.
(A) Mice reconstituted with a mixture of 1 x106 α7lin+ and 1×106 α7lin– cells were assessed at 8 weeks for blood cells of donor origin (CD45.2) (A, left panel). At 10 weeks post-reconstitution mice were injected with 25 µg of LPS i.p. and the blood of LPS injected mice was analyzed 48 hours later (A, right panel). (B) Isotype control antibodies used for the detection of intracellular cytokines. (C) Flow cytometry of intracellular stained cytokines in spleen cells of α7lin−/lin+ reconstituted mice 48 hours post-LPS challenge. Results are expressed as cytokine positive cells in the total spleen cell population (top row), CD11b positive and cytokine positive cells (C, middle row), or B220 positive and cytokine positive cells (C, bottom row). The cytokines examined were murine TNFα, IL-12/23(p40), IL-6, and IL-10. Results have been repeated 3 times.