Downregulation of yidC in Escherichia coli by Antisense RNA Expression Results in Sensitization to Antibacterial Essential Oils Eugenol and Carvacrol
Figure 1
Effect of yidC antisense expression on growth of E. coli.
E. coli DH5α harbouring pHN678 (negative control for antisense expression), pHN678-yidC or pHN678-murA (positive control for antisense expression) were cultured in LB broth supplemented with chloramphenicol (30 µg/ml). The overnight cultures were diluted hundred fold in fresh LB broth supplemented with chloramphenicol (30 µg/ml) of which 5 µl was spotted on LB chloramphenicol plates without (upper panel) and with IPTG (lower panel). There was no change observed in the phenotype of E. coli DH5α harbouring vector (pHN678) without any insert upon induction with IPTG after 14 hrs of incubation at 37°C. However, the growth of E. coli DH5α cells expressing antisense of murA and yidC showed growth suppression upon induction.