P-Selectin Glycoprotein Ligand-1 Forms Dimeric Interactions with E-Selectin but Monomeric Interactions with L-Selectin on Cell Surfaces
Figure 5
Binding curves of neutrophil L-selectin interacting with (s)PSGL-1.
Adhesion frequency vs. contact duration plots of neutrophils constitutively expressing native L-selectin interacting with RBCs coated with matched densities of sPSGL-1 (square) or PSGL-1 (triangle) at 59 µm−2 (A) or 25 µm−2 (B). Control for nonspecific adhesion was measured using RBCs coated with the capture antibody PL2 without incubation with (s)PSGL-1. The differences in the first three data points in (A) between sPSGL-1 and PSGL-1 interacting with L-selectin are not significant as assessed by the Student-t test (p = 0.32, 0.15, and 0.75).