Cfr-Mediated Linezolid-Resistance among Methicillin-Resistant Coagulase-Negative Staphylococci from Infections of Humans
Figure 1
Identification and characterization of the pSS-02-like cfr-carrying plasmid found in the four MRCoNS isolates in comparison to the original plasmid pSS-02.
(A) S1 nuclease-PFGE (B) Southern blot hybridization with the cfr probe. Lane 1:S. haemolyticus 09D279, Lane 2: S. cohnii 09D253, Lane 3: S. cohnii 09D363; Lane 4: S. cohnii NDM113; Lane 5: RN4220 + pSS-02. (C) Restriction digests of the pSS-02-like plasmid extracted from transformants originating from S. haemolyticus 09D279 (lanes 1, 3, 5, and 7) and the original plasmid pSS-02 extracted from transformants originating from S. saprophyticus 2-87 (lanes 2, 4, 6, and 8). The restriction enzymes used to digest the plasmids are indicated above the respective lanes.