Mangiferin, a Natural Xanthone, Protects Murine Liver in Pb(II) Induced Hepatic Damage and Cell Death via MAP Kinase, NF-κB and Mitochondria Dependent Pathways
Figure 8
Immunoblot analysis of NF-κB, IκBα and IKKα proteins in response to Pb(II) and mangiferin treatment, both in liver and hepatocytes.
Hepatocytes were treated with Pb(II) (30 µg/mL) and mangiferin (50 µg/mL) for 2 h. β actin was used as an internal control Cont: normal control, Mang: treated with mangiferin, Pb(II): administered with Pb(II), Pb(II)+Mang: Mangiferin treated post to Pb(II) administration, Pb(II)&Mang: Simultaneous exposure of Pb(II) and mangiferin in hepatocytes. Data are mean ± SEM, for 6 sets per group and were analyzed by one-way ANOVA, with Student-Newman-Keuls post hoc tests. Differences were attributed at p<0.05, and homogeneous subgroups share common superscripted letters.