In Vitro Characterization of Echinomycin Biosynthesis: Formation and Hydroxylation of L-Tryptophanyl-S-Enzyme and Oxidation of (2S,3S) β-Hydroxytryptophan
Figure 8
LC-MS analysis of the reaction catalyzed by Qui17.
‘No protein’ represents the sample of negative control lacking Qui17.The reaction was terminated after incubation of 30 min, 60 min, 90 min respectively. The peaks I and II are (2S,3S) β-hydroxytryptophan and (2S,3R) N-formyl-β-hydroxykynurenine. The chemical structure of each compound is shown.