Cathepsin E Is a Marker of Gastric Differentiation and Signet-Ring Cell Carcinoma of Stomach: A Novel Suggestion on Gastric Tumorigenesis
Figure 1
(A) Expression of E-cadherin, LI-cadherin, MUC5AC, MUC6, MUC2, vimentin, CTSE, CTSD, CTSB, CTSL, and GAPDH (internal control) mRNAs in a panel of 32 human cancer cell lines.
20 gastric, 10 colorectal, and 2 non-gastrointestinal cell lines (HeLa-S3 and MDA-MB435) were analyzed by RT-PCR. (B) Expression of CTSE protein in 13 gastric, 5 colorectal, and 2 non-gastrointestinal cancer cell lines analyzed by Western blotting. (C) RT-PCR detecting CTSE mRNA in 5 gastric cancer cells treated with 5-Aza-dC and/or TSA for 48 hours. (D) RT-PCR detecting CTSE mRNA in gastric (AGS, MKN-1, SH-10-TC), colorectal (WiDr, Lovo, SW480, DLD-1), and breast cancer (MDA-MB435) cell lines stably transduced with retroviral vector encoding cdx2, gli1, gli3, or sox2 genes.