Biochemical and Mutational Analysis of a Novel Nicotinamidase from Oceanobacillus iheyensis HTE831
Figure 1
Multiple sequence alignment for O. iheyensis nicotinamidase (OiNIC) and related nicotinamidases.
ESPript outputs [48] obtained with the crystallized nicotinamidase sequences retrieved from Uniprot database and later aligned with CLUSTAL-W [47]. Sequences are grouped according to similarity. PDB codes were 3O90 for S. pneumoniae nicotinamidase (SpNIC), 1ILW for P. horikoshii nicotinamidase (PhPncA), 3PL1 for M. tuberculosis nicotinamidase (MtPncA), 2WT9 for A. baumanii nicotinamidase (AbPncA), 2H0R for S. cerevisiae nicotinamidase and 2R3J for Leishmania infantum nicotinamidase. Residues strictly conserved across nicotinamidase enzymes have a dark background. Symbols above blocks of sequences represent the secondary structure, springs represent helices and arrows represent β-strands. The residues forming the active site are indicated by triangles. Residues involved in the coordination of the metal ion are indicated by stars and residues forming the cis-peptide bond are indicated by diamonds.